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When the first coat is dry...

(Try to cover all your white canvas spots on the first coat if you can.)

 
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Cover the whole painting again.

Think about starting in a place that will keep your hands out of wet paint. (At the top for landscapes.)

 
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Try for more accurate color

The second coat should be richer, more vibrant, easier to match than on the first coat. Sometimes a color that's off on the first coat shows through and makes a more interesting second coat. 

 
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Aim for more contrast

Odds are you'll need to push for darker darks to achieve the illusion of more volume. 

 
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Highlights and reflections

In addition to darkening the shadows, don't be shy about making your lights light. Get more detail in general. 

 
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Final touches later

You're still focused on a general (albeit more detailed) covering of the whole painting. You can come in with polishing touches later.